Isolation And Identification Of Fungi Associated With Local Fruits And Vegetables From Kochi And Mattancherry Market, India
Kavya Vinayachandran, Arpitha M R, Manju V Subramanian*, Smitha N R, Vineeth Kumar T V, Princy Varghese
Department of Zoology, The Cochin College, Kochi - 682002, Kerala; Affiliated to Mahatma Gandhi University, Kerala.
The study was carried out to examine the fungi associated with some fruits and vegetables in mattancherry and kochi market also to screen the isolated fungi for amylytic and cellulolytic activity. Ten fungal pathogens were isolated which caused spoilage of vegetables. The isolation of fungi from different vegetable samples was done by sterilization technique and spread plate technique. Samples were plated out on potato dextrose agar (PDA) medium and incubated at 28°C±2°C. Resulting growth was microscopically screened for fungal species. The isolated fungi were identified on the basis of macromorphological and micromorphological characteristics. The following morphological characteristics viz. colony growth, presence or absence of aerial mycelium, colony color, presence of wrinkles and furrows, pigment production etc. was recorded. Ten different fungal strains such as Aspergillus flavus species, Aspergillus niger species, Curvularia species, Fusarium species, Mucor species, Mucor mucedo species, Pencillum species, Rhizopus species, Rhizomucor species and Syncephalastrum racemosum species were isolated and identified. The five fungal isolates were screened for extracellular amylase and cellulose but only some possess cellulosic activity. All the fungal species possess degrading properties and secrete extracellular enzymes. This study confirms the isolated filamentous fungi from vegetable samples provide a basic data for investigation on degradative properties produced by extracellular enzymes. This study has provided useful information about the toxigenic fungi associated with local vegetables which may affects the human health. Spoiled fruits should be sorted and eliminated to avoid toxins usually associated with the growth of fungi
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How to cite this article
Kavya Vinayachandran, Arpitha M R, Manju V Subramanian, Smitha N R, Vineeth Kumar T V, Princy Varghese, 2020. Isolation And Identification Of Fungi Associated With Local Fruits And Vegetables From Kochi And Mattancherry Market, India. Bioscience Discovery, 11(4):239-248.
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