In Vitro Studies In Medicinal Plant Bacopa monnieri L.
Pramod Fuke, Sangeeta Ahuja1 and Narayan Pandhure
1Dept of Botany, Sir Sayyed College, Aurangabad
Tissue Culture Laboratory, Department of Botany,
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004
The present investigations micropropagation studies have been carried out in highly medicinal plant Bacopa monnieri L.. Effect of auxins (IAA, NAA & 2, 4-D) and cytokinins (BA, KIN & TDZ) on callus induction and IAA and TDZ on root induction were assessed. Callus induction on Murashige and Skoog’s medium (MS) supplemented with NAA, 2,4-D and TDZ at various combinations/concentrations were also investigated. The auxiliary buds formed from both leaf and nodal explants on hormone free MS medium within 9 d. Growth hormones at different combinations brought out remarkable variations in shoot, root and callus induction.
Keywords: Bacopa monnieri L., growth hormones, auxiliary shoot proliferation, transplantation.
Anilkumar M, Mathew SK, Mathew P, John S, Deepa KP and Kiran VS, 2005. In vitro shoot multiplication in Ocimum basilicum L. Plant Cell Biotechnol. Mol. Biol. 6:73-76.
Anilkumar M and Sajeevan RS, 2005. Micropropagation of Musa acuminata colla. Plant Cell Biotechnol. Mol. Biol. 6:159-162.
Ekka RN and Dixit VK, 2007. Ethno-pharmacognostical studies of medicinal plants of Jashpur district, Chattisgarh. Int. J. Green Phar. 1:2-4.
Ignacimuthu S, 1997. Plant Biotechnology, Oxford and IBH publishing Co. Pvt. Ltd, p. 180.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
Nagaraja YP, Krishna V and Maruthi KR, 2003. Rapid micropropagation of Andrographis alata Nees. through leaf callus culture. Plant Cell Biotechnol. Mol. 4:117- 124.
Negrutiu I, Jacobs N and Caboche M, 1984. Theor. Appl. Genet. 67: 289-304.Rahman MH, Ramawat KG, 2003. Plant Biotechnology, S. Chand and Co. pp:1-37.
Sehrawat AR, Sanjogta U and Anita P, 2001. In vitro culture and multiplication of Rauwolfia serpentine – a threatened medicinal plant. Crop Res. 22:68-71.
Singh S, Ray BK, Mathew S, Buragohain P, Gogoi J, Gogoi S, Sharma BK and Deka PC, 1999. Micropropagation of a few important medicinal plants. Ann. Biol. 15:1-7.
Thejavathi DH, Sowmya R and Shailaja KS, 2001. Micropropagation of Bacopa monnieri using shoot tip and nodal explants. J. Trop. Med. Plants. 2:39-45.
Tiwari V, Singh BD and Tiwari KN, 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L.) Wettst.]. Plant Cell Rep. 17:538-543.
Tiwari V, Tiwari KN and Singh BD, 2001. Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tiss. Org. Cult. 66:9-16.
How to cite this article
Pramod Fuke, Sangeeta Ahuja1 and Narayan Pandhure 2020. In Vitro Studies In Medicinal Plant Bacopa monnieri L.. Bioscience Discovery, 11(4):195-198.
Google Scholar citation: https://scholar.google.co.in/citations?user=vPzEyC8AAAAJ&hl=en