Bioscience Discovery, Volume 11, Number 4, Page Number 191-251, Month & Year: Oct. - 2020

Tissue Culture Studies In Raulfai tetraphylla L.

Shilpa Chavan, Afreen Attar, Ganesh Kolekar and Narayan Pandhure

Tissue Culture Laboratory, Department of Botany,
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001, India.

Article Info



Received: 10-08-2020,
Revised: 10-09-2020,
Accepted: 16-09-2020

Rauvolfia tetraphylla L. is an endangered plant, known for its medicinal properties. It contains various indole group alkaloids with reserpine most prominent among them. Reserpine is a hypotensive agent that is in great demand for the modern pharmaceutical industries. In the present work, leaf explants were induced to produce calli by using the phytohormones 2,4-D, NAA, IBA and IAA. Among these, 9 μM 2,4-D was found suitable for maximum callus induction (95%). The calli produced in this hormone concentration were subjected to NaCl salt treatment (0, 25, 50, 75 and 100 mM) and the effect of salinity on callus growth and reserpine accumulation was observed. The callus growth was normal up to 50 mM concentration of NaCl and there was a reduction in growth of the calli at 75 mM salt treatment whereas at 100 mM concentration complete cessation of callus growth was noticed. An increase in reserpine accumulation was noticed with increases measured up to 75 mM NaCl concentration.

Keywords: Callus, NaCl, Reserpine, 2,4-D, Rauvolfia tetraphylla L. doi: 10.2306/scienceasia1513-1874.2006.32.417

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How to cite this article

Shilpa Chavan, Afreen Attar, Ganesh Kolekar and Narayan Pandhure, 2020. Tissue Culture Studies In Raulfai tetraphylla L. Bioscience Discovery, 11(4):208-211.

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