Bioscience Discovery, Volume 11, Number 2, Page Number 67-110, Month & Year: April - 2020

Studies on Recycling of Used Agar for In Vitro Propagation of Banana CV. Grand Naine

Sherkar Sandip Harishchandra, Anita Sharma, Sherkar H.D. and Pankaj Rathod

Genuine Biosciences, Gut No 121/2, Sambhaginagar, Dist-Osmanabad MH-43 501.

Article Info

 

Abstract

Received: 20-12-2019,
Revised: 26-02-2020,
Accepted: 27-02-2020

The nutrient media (Murashige and Skoog 1962) is widely used in micropropagation of banana cv. Grand naine and discarded after the use. A simple, efficient, economical and highly reproducible protocol is developed for the in vitro propagation of banana. In the present study we collected used agar, recycled and used to prepare MS media. It was found that the control media and MS media supplemented with 4 mgs BAP and 10 mgs AdSO4 along with used agar media showed good shoot multiplication (1:2.51 and 1:2.34) and shoot height. All the three media MS + IBA (control), MS + used agar + IBA, and used agar alone + IBA showed 100 percent rooting response. The average length of roots is almost similar (40.567 mm, 39.567 mm, and 38.333 mm).

Keywords: Agar-agar, used agar media, recycling, re-melting.

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How to cite this article

Sherkar Sandip Harishchandra, Anita Sharma, Sherkar H.D. and Pankaj Rathod,  2020. Studies on Recycling of Used Agar for In Vitro Propagation of Banana CV. Grand Naine. Bioscience Discovery, 11(2):90-94.

Google Scholar citation: https://scholar.google.co.in/citations?user=vPzEyC8AAAAJ&hl=en

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